Fractional distillation is the process of separating two or more miscible liquids by distillation. The distillate being collected in fractions boiling at different temperatures. The separation of two liquids by fractional distillation depends on the differences in their boiling points. A simple Fractionating column is a long vertical glass tube filled with glass beads. It can be regarded as an arrangement for providing different temperature zones inside it, the highest temperature being at the bottom of the column and lowest temperature near its top. The Fractionating column is fitted in the neck of the distillation flask containing the mixture of liquids to be separated.