Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) into a bacterium like E. coli?
• DNA cloning is the method of producing multiple identical copies of specific DNA template.
• It involves the use of a vector to carry the specific, foreign DNA fragment into the host cell.
• The mechanism of cloning and transfer of gene for growth hormone into E.coli is depicted below.
1