The process of nitrogen-fixation by bacteria does not take place in the presence of
Biological nitrogen fixation (BNF) occurs when atmospheric nitrogen is converted to ammonia by an enzyme called a nitrogenase. The reaction for BNF is as follows
N2+ 8 H+ + 8 e−→ 2 NH3 + H. The process is coupled to the hydrolysis of 16 equivalents of ATP and is accompanied by the co-formation of one molecule of H2