Given below are the steps to test the presence of proteins in a food item:

(i) Take a small quantity of the food item in a test tube, add 10 drops of water to it shake it.


(ii) Make a paste or powder of food to be tested.


(iii) Add 10 drops of caustic soda solution to the test tube and shake well.


(iv)Add 2 drops of copper sulphate solution to it.


Which of the following is the correct sequence of the steps?

The presence of protein in a food material is tested on the basis of a reaction between proteins and alkaline solution of copper sulphate, that gives a violet color.


Test involves the sequence in ii, i, iii, iv

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