Separation of 2 immiscible liquids is done using a separating funnel. The above figure shows a separating funnel clamped to a stand. In this case the mixture to be separated contains oil and water.

Immiscible liquids do not mix well and have different densities. When the mixture is left undisturbed, the denser liquid comes to the bottom of the funnel, and the lighter liquid stays on top of it. Now by operating a stopcock carefully, one can transfer the denser liquid (here, water) to the conical flask. The stopcock is closed when water is completely transferred from the funnel. The separation is done multiple times to ensure complete separation of the two components. Oil remains in the funnel and water is in a conical flask.